Vectors for RNAi technology in poplar.
Identifieur interne : 004135 ( Main/Exploration ); précédent : 004134; suivant : 004136Vectors for RNAi technology in poplar.
Auteurs : S. Meyer [Allemagne] ; K. Nowak ; V K Sharma ; J. Schulze ; R R Mendel ; R. H NschSource :
- Plant biology (Stuttgart, Germany) [ 1435-8603 ]
Descripteurs français
- KwdFr :
- Amorces ADN (génétique), Glucuronidase (génétique), Gènes rapporteurs (MeSH), Interférence par ARN (MeSH), Populus (génétique), Protéines recombinantes (génétique), Rhizobium (génétique), Séquence nucléotidique (MeSH), Transformation génétique (MeSH), Vecteurs génétiques (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA Primers, Glucuronidase, Recombinant Proteins.
- genetics : Populus, Rhizobium.
- Base Sequence, Genes, Reporter, Genetic Vectors, Plants, Genetically Modified, RNA Interference, Transformation, Genetic.
Abstract
The potential of double-stranded RNA interference (RNAi) technology was studied for down-regulation of gene expression in poplar. A set of vectors was constructed generating RNAs capable of duplex formation of sequences specific for the beta-glucuronidase (GUS) reporter gene system. These gene cassettes are driven by the CaMV-35S promoter. To address the question of gene silencing, we tested the functionality of these vectors, both in transient assays by transforming protoplasts with the RNAi constructs, and in stably transformed GUSexpressing poplar plants. Agrobacterium-mediated transformation of those GUS-expressing plants with a GUS-specific RNAi construct showed a strong down-regulation of the reporter gene. From these results we conclude that RNAi is also functional in poplar.
DOI: 10.1055/s-2004-815729
PubMed: 15095140
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Nowak</name></author><author><name sortKey="Sharma, V K" sort="Sharma, V K" uniqKey="Sharma V" first="V K" last="Sharma">V K Sharma</name></author><author><name sortKey="Schulze, J" sort="Schulze, J" uniqKey="Schulze J" first="J" last="Schulze">J. Schulze</name></author><author><name sortKey="Mendel, R R" sort="Mendel, R R" uniqKey="Mendel R" first="R R" last="Mendel">R R Mendel</name></author><author><name sortKey="H Nsch, R" sort="H Nsch, R" uniqKey="H Nsch R" first="R" last="H Nsch">R. H Nsch</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004 Jan-Feb</date><idno type="RBID">pubmed:15095140</idno><idno type="pmid">15095140</idno><idno type="doi">10.1055/s-2004-815729</idno><idno type="wicri:Area/Main/Corpus">004278</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004278</idno><idno type="wicri:Area/Main/Curation">004278</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004278</idno><idno type="wicri:Area/Main/Exploration">004278</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Vectors for RNAi technology in poplar.</title><author><name sortKey="Meyer, S" sort="Meyer, S" uniqKey="Meyer S" first="S" last="Meyer">S. Meyer</name><affiliation wicri:level="1"><nlm:affiliation>Institut für Pflanzenbiologie, Technische Universität Braunschweig, Braunschweig, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Pflanzenbiologie, Technische Universität Braunschweig, Braunschweig</wicri:regionArea><wicri:noRegion>Braunschweig</wicri:noRegion><wicri:noRegion>Braunschweig</wicri:noRegion><wicri:noRegion>Braunschweig</wicri:noRegion></affiliation></author><author><name sortKey="Nowak, K" sort="Nowak, K" uniqKey="Nowak K" first="K" last="Nowak">K. Nowak</name></author><author><name sortKey="Sharma, V K" sort="Sharma, V K" uniqKey="Sharma V" first="V K" last="Sharma">V K Sharma</name></author><author><name sortKey="Schulze, J" sort="Schulze, J" uniqKey="Schulze J" first="J" last="Schulze">J. Schulze</name></author><author><name sortKey="Mendel, R R" sort="Mendel, R R" uniqKey="Mendel R" first="R R" last="Mendel">R R Mendel</name></author><author><name sortKey="H Nsch, R" sort="H Nsch, R" uniqKey="H Nsch R" first="R" last="H Nsch">R. H Nsch</name></author></analytic><series><title level="j">Plant biology (Stuttgart, Germany)</title><idno type="ISSN">1435-8603</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>DNA Primers (genetics)</term><term>Genes, Reporter (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>Glucuronidase (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>RNA Interference (MeSH)</term><term>Recombinant Proteins (genetics)</term><term>Rhizobium (genetics)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (génétique)</term><term>Glucuronidase (génétique)</term><term>Gènes rapporteurs (MeSH)</term><term>Interférence par ARN (MeSH)</term><term>Populus (génétique)</term><term>Protéines recombinantes (génétique)</term><term>Rhizobium (génétique)</term><term>Séquence nucléotidique (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Vecteurs génétiques (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term><term>Glucuronidase</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term><term>Glucuronidase</term><term>Populus</term><term>Protéines recombinantes</term><term>Rhizobium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Genes, Reporter</term><term>Genetic Vectors</term><term>Plants, Genetically Modified</term><term>RNA Interference</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Gènes rapporteurs</term><term>Interférence par ARN</term><term>Séquence nucléotidique</term><term>Transformation génétique</term><term>Vecteurs génétiques</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The potential of double-stranded RNA interference (RNAi) technology was studied for down-regulation of gene expression in poplar. A set of vectors was constructed generating RNAs capable of duplex formation of sequences specific for the beta-glucuronidase (GUS) reporter gene system. These gene cassettes are driven by the CaMV-35S promoter. To address the question of gene silencing, we tested the functionality of these vectors, both in transient assays by transforming protoplasts with the RNAi constructs, and in stably transformed GUSexpressing poplar plants. Agrobacterium-mediated transformation of those GUS-expressing plants with a GUS-specific RNAi construct showed a strong down-regulation of the reporter gene. From these results we conclude that RNAi is also functional in poplar.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15095140</PMID><DateCompleted><Year>2004</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2008</Year><Month>01</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1435-8603</ISSN><JournalIssue CitedMedium="Print"><Volume>6</Volume><Issue>1</Issue><PubDate><MedlineDate>2004 Jan-Feb</MedlineDate></PubDate></JournalIssue><Title>Plant biology (Stuttgart, Germany)</Title><ISOAbbreviation>Plant Biol (Stuttg)</ISOAbbreviation></Journal><ArticleTitle>Vectors for RNAi technology in poplar.</ArticleTitle><Pagination><MedlinePgn>100-3</MedlinePgn></Pagination><Abstract><AbstractText>The potential of double-stranded RNA interference (RNAi) technology was studied for down-regulation of gene expression in poplar. A set of vectors was constructed generating RNAs capable of duplex formation of sequences specific for the beta-glucuronidase (GUS) reporter gene system. These gene cassettes are driven by the CaMV-35S promoter. To address the question of gene silencing, we tested the functionality of these vectors, both in transient assays by transforming protoplasts with the RNAi constructs, and in stably transformed GUSexpressing poplar plants. Agrobacterium-mediated transformation of those GUS-expressing plants with a GUS-specific RNAi construct showed a strong down-regulation of the reporter gene. From these results we conclude that RNAi is also functional in poplar.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Meyer</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institut für Pflanzenbiologie, Technische Universität Braunschweig, Braunschweig, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nowak</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Sharma</LastName><ForeName>V K</ForeName><Initials>VK</Initials></Author><Author ValidYN="Y"><LastName>Schulze</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Mendel</LastName><ForeName>R R</ForeName><Initials>RR</Initials></Author><Author ValidYN="Y"><LastName>Hänsch</LastName><ForeName>R</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biol (Stuttg)</MedlineTA><NlmUniqueID>101148926</NlmUniqueID><ISSNLinking>1435-8603</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="Y">RNA Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>4</Month><Day>20</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>5</Month><Day>20</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>4</Month><Day>20</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15095140</ArticleId><ArticleId IdType="doi">10.1055/s-2004-815729</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="H Nsch, R" sort="H Nsch, R" uniqKey="H Nsch R" first="R" last="H Nsch">R. H Nsch</name><name sortKey="Mendel, R R" sort="Mendel, R R" uniqKey="Mendel R" first="R R" last="Mendel">R R Mendel</name><name sortKey="Nowak, K" sort="Nowak, K" uniqKey="Nowak K" first="K" last="Nowak">K. Nowak</name><name sortKey="Schulze, J" sort="Schulze, J" uniqKey="Schulze J" first="J" last="Schulze">J. Schulze</name><name sortKey="Sharma, V K" sort="Sharma, V K" uniqKey="Sharma V" first="V K" last="Sharma">V K Sharma</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Meyer, S" sort="Meyer, S" uniqKey="Meyer S" first="S" last="Meyer">S. Meyer</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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